Indole is a hazardous substance existing in the curl oil, which released to the environment by various industrial activities; thus, decreasing its ratio from nature seems to be essential. In this study, a facial pathway for loading Cu(II) particles on MCM-41 mesoporous material was introduced as an efficient catalyst for indole oxidation. The obtained catalyst was characterized by XRD, FESEM, EDS, TEM and BET/BJH techniques. Based on Taguchi method, indole oxidation via prepared catalyst at mild condition was evaluated. By applying this method, the operating factors including Indole initial concentration (mg/L), pH value and the mass of catalyst (g) were optimized. It was found that pH plays the most crucial role in indole oxidation, on the other word, in alkaline environment; indole oxidation was happened with higher yield.

The regioselectivity of the [3+2] cycloaddition reactions between benzonitrile N-oxide as three-atom component and two series of para-substituted β-nitrostyrene analogues was analysed in the framework of
a Molecular Electron Density Theory. All of the considered processes were found to be initiated by the attack of the most nucleophilic oxygen atom in the benzonitrile N-oxide on the most electrophilic carbon atom (C_α) in the nitroalkenes. This type of interaction favours the formation of 4-nitro-substituted
Δ²-isoxazolines.

New series of mononuclear octahedral Ru(III) complexes of general formula [RuL^1-3Cl]Cl₂ (1-3) where L = dianion of Schiff bases namely: 2,2'-(((1E,1'E)-thiophene-2,5-diylbis(methaneylylidene))bis(azaneylylidene))diphenol (H₂L¹), 2,2'-(((1E,1'E)-thiophene-2,5-diylbis(methaneylylidene))bis(azaneylylidene))dibenzenethiol (H2L2) and 2,2'-(((1E,1'E)-thiophene-2,5-diylbis(methaneylyli-dene))bis(azaneylyli-dene))-dibenzoic acid (H₂L³), respectively are synthesized and characterized by different physico-chemical techniques. The results of interaction of complexes with CT-DNA supported that, the Ru (III) complexes bind to DNA via an intercalative mode. The interaction has also been investigated by gel electrophoresis. Interestingly, it was found that all Ru(III) complexes cleave super coiled (SC) pUC19 plasmid DNA efficiently in the absence of an external agent. Further, the antiproliferative activity of the complexes on Human Cervical Cancer Cells (HeLa) and Human Breast Cancer Cells (MCF-7) were evaluated by MTT assay, which revealed that, all the complexes showed more intense inhibition against HeLa and MCF-7 cell lines, particularly Ru(III) complex (3) attenuated the strongest proliferation, allowing its use as chemotherapeutic agent for cancer treatment.

A new series of 4-amino-4H-1,2,4-triazole derivatives, 3a-f and 5a-f were synthesized by using various aryl aldehydes and ketones. FT-IR, ¹H NMR, ¹³C NMR and mass spectral studies were characteristic of the synthesized compounds. These new compounds were screened in male wistar rats and compared with the standard drug for their anticonvulsant activity against maximal electroshock seizure (MES) model. Compounds 3b and 5d of this sequence were found to be the most active. The same compound did not demonstrate neurotoxicity at the administered maximum dose (100 mg / kg) and these compounds showed good protection compared to other compounds.

A synthesis of 2-amino-4-(R)-7,7-dimethyl-5-oxo-5,6,7,8-tetrahydro-4H-chromene-3-carbonitrile derivatives (4a-l) in single flasks containing aromatic aldehydes (1a-l), malononitrile (2), and dimedone (3) by using green catalyst, extract of orange without solvent under ultrasound irradiations and reflux condition. Synthesis of 2-amino-4-(R)-7,7-dimethyl-5-oxo-5,6,7,8-tetrahydro-4H-chromene-3-carbonitrile derivatives (4a-l) show potent in-vitro anti-inflammatory activity. All synthesized compounds were characterized by FTIR, ¹H NMR, ¹³C NMR and mass spectroscopic data & elemental analysis.

Epithelial sodium channels (ENaC) is a protein that plays a role in maintaining sodium levels and can be used as a biomarker of hypertension. This study aims to develop an electrochemical immunosensor for detecting ENaC using a screen-printed carbon electrode (SPCE) which is modified with reduced graphene oxide (RGO). The performance of RGO enhanced to provide covalent bonds between the -COOH residues at RGO and the terminal -NH2 of anti-ENaC. While anti-ENaC and ENaC decrease the peak of ferric cyanides current because of the resistance effect. The optimum concentration of anti-ENaC was 1.0 µg/mL for 30 min incubation time. The various concentration of ENaC has a linear relationship with the decreasing of ferric cyanide peak current in the range of 0.01-1.5 ng/mL, and the limit of detection found at 0.198 ng/mL. The low-level exposure of ENaC warrants the realization of a point-of-care device for early detection of hypertension in urine samples.

In order to investigate the isomerization and conversion mechanism of the advantageous and widely used nonsteroidal anti-inflammatory medicine flurbiprofen, the hybrid density functional theory was applied. According to the results, the rearrangement reaction of (R)-flurbiprofen to its (S)-enantiomer happens in a [1,3]-hydrogen shifts with inversion of configuration at chiral center C14. From the calculated energies, it can be understood that the rate-limiting step in the flurbiprofen isomerization is the excitation of (R)-flurbiprofen methyl ester in its initial form to the first excited singlet state S1 at λ=243.91 nm. we studied this process by scanning the C14-H17 distance for the excited singlet to get more information about the process of isomerization occurring upon excitation. The results of calculations demonstrated that the isomerization process should pass through a ~71 kcal/mol barrier. The (S)-flurbiprofen methyl ester is more photostable than its related (R)-enantiomer. This issue can be attributed to the -1.50 kcal/mol of thermodynamic stability of the (S)-flurbiprofen methyl ester.

Contamination of soils by trace elements is a worldwide concern and has negative effects on environmental sustainability. Geochemical assessment of soils using appropriate indicators and pollution indices has received much attention in recent years in efforts to rehabilitate this resource. This study quantified pollution of soils by trace elements at the Roundhill landfill, South Africa using indices and multivariate statistics. Soils were collected and assayed for trace metals using x-ray fluorescence. Pollution indices classified soil contamination levels while multivariate statistical analysis was conducted using principal component and cluster analyses. Findings showed that concentrations of all elements decreased with increasing distance from the landfill. Low to extremely high pollution was evident in all soils and Cr had the highest values compared to other elements. Negative correlation and weak clustering of Cr and Cd was associated with different wastes disposed at the landfill. Reported pollution in soils was associated with the influence of landfill leachate in the investigated area.

A series of fourteen chalcone was synthesized via. Claisen–Schmidt condensation between substituted 2- hydroxyl acetonaphthones and substituted benzaldehyde in presence of tripotassium phosphate (K3PO4) catalyst. The reaction was carried out by conventional method using 2-methoxyethanol. The procedure is simple and efficient in terms of reaction time, easy workup and isolation of products and yields. In-vitro all these synthesized compounds were screened and evaluated for the cytotoxic and antimicrobial activity. It was found that these compounds had significant cytotoxic activity in comparison with standard 5-flurouracil. The compounds 3a, 3b, 3h, 3f and 3l were screened by MTT assay against liver cancer cell line-HepG2. Among these, the compound 3b and 3c showed LC50 values of 997.14 μM/ml and 284.13 μM/ml., respectively. The remaining compounds did not display the LC50 values. The compound 3l displayed the strongest cytotoxic activities with IC50 value of 91.85 μg/ml against liver cancer cell line. The Chalcone 3a, 3f, 3h and 3e demonstrated excellent antimicrobial activity and the remaining were moderately active against tested pathogens. The antimicrobial effects of all the tested compounds are due to the presence of pharmacological active substituent in the basic nucleus of Chalcones. Therefore, the present study leads to the development of new class of anticancer and antimicrobial inhibitory candidates.

N-Heterocyclic carbenes are widely used in organic reactions and coordination chemistry. In the present study, 2,3-dihydro-1,3-diisopropyl-4,5-dimethylimidazol-2-ylidene (1) is reacted with diphenyl disulfide, methyl phenyl disulfide, and bis(methylsulfonyl)methane to yield target compounds 5, 6, and 7 respectively. Structures of these compounds are well established using nuclear magnetic resonance, mass spectrometry and elemental analysis. Possible reaction mechanisms are proposed.