Novel membrane sensor for determination of lamotrigine in pharmaceuticals and urine

Abstract: Lamotrigine (LMT), chemically known as [6-(2,3-dichlorophenyl)-1,2,4-triazine-3,5-diamine], is a broad spectrum antiepileptic drug, used as monotherapy and as an adjunct with other antiepileptic drugs for treatment of partial and generalized toxic-clonic seizures. It is used to treat neurological lesions and as a tranquilizer. A selective electrochemical membrane sensor has been developed and validated for determination of LMT. The membrane constructed using LMT and molybdophosphoric acid in THF and PVC is applicable for the detection of 5 × 10^-4 to 9 × 10^-3 M LMT in the pH range between 4.6 and 5.8 with the Nernstian slope of 57.14±1 mV/decade. The regression coefficient value of 0.9932 showed a good linear correlation between the concentrations of LMT and measured cell potentials. The limits of detection (LOD) and quantification (LOQ) values for the fabricated sensor were 1.3 × 10^-5 and 4 × 10^-5 M LMT, respectively. Various experimental conditions were optimized to reach the effective performance characteristics of the sensor. The effect of various cations, anions and organic species on the performance of sensor was studied by following standard-addition procedure. The results revealed no such variations due to presence of foreign ions or species. The fabricated sensor was subjected to validation to check accuracy, precision, robustness and ruggedness. The mean accuracy for determination of LMT was found to be 99.16%. The developed sensor was successfully used to determine LMT in tablets and in spiked human urine.

How to cite this paper

 Rajendraprasad, N. (2019). Novel membrane sensor for determination of lamotrigine in pharmaceuticals and urine.Current Chemistry Letters, 8(2), 87-96.

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