Digitization technology has developed in the preservation of cultural heritage, especially ancient manuscripts. In this context, image retrieval methods allow for efficient access to the information contained in ancient manuscripts. Optimization techniques play a role in building an effective image retrieval method. This paper presents a systematic literature review that focuses on the role of optimization in digital image retrieval methods for preserving ancient manuscripts as part of cultural heritage preservation. This paper is organized based on the following research questions: (1) What are the research objectives regarding optimization in digital image retrieval of ancient manuscripts?; (2) What is the role of optimization in image retrieval methods for the preservation of ancient manuscripts?; and (3) How have existing studies determined the formulation of novel and new research? This study involved searching articles through Scopus, Dimensions, Science Direct, and Google Scholar databases and using bibliometric analysis to visualize research themes and trends. The results of the literature review show that research related to the role of optimization in the digital image retrieval method of ancient manuscripts is still open and this paper can be used as a reference for further research on this topic.