Transportation is one of the most important functions of logistics management because of its impact on both the level of customer service provision and the organization’s cost structure. The study explores the transportation cost optimization strategies and how they affect the supply chain performance of Shea nuts in the Agro-processing industries in Ghana. The quantitative study design was adopted and utilized to solicit data. With a sample size of 204 respondents. Data was analyzed using multiple linear regression in Statistical Package for Social Sciences (SPSS v20). The results revealed interesting findings in the supply chain within the institution as it seeks to assert that transportation is seen to be effective and contribute highly to firms output when Agro companies use outsourcing and pooling as means of transport. From the results, the supply chain correlated positively and significantly with outsourcing and pooling as transport optimization practices in Agro firms supply chain. This paper adds to the existing literature on the supply chain systems of Agro processing firms but departs from previous studies which seek to concentrate not on the transport optimization of the Agro processing firms but rather on the challenges, production, and profit maximization of these firms. Our study is unique unlike any other research previously authored as it seeks to reveal the option of transport optimization that is cost effective and efficiently productive for Agro processing firms to adopt.